Scientists from the Department of Optics, Faculty of Science, Palacký University, have introduced a new microscopy technique that changes the rules of the game in cell imaging. It will enable fast, contactless and non-invasive analysis of cells with high resolution. The results were published in the prestigious scientific journal Optica.
Their method allows capturing the internal structures of cells with a resolution of 250 nanometers and a phase accuracy of better than 2 nanometers. It can therefore capture a change in the optical path corresponding to the size of a few molecules. In addition, it works with conventional incoherent illumination, thus eliminating disturbing artifacts and enabling sharper and faster imaging. Thanks to the new optical arrangement, the method is resistant to vibration and ideal for long-term experiments.
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